Which infectious agent is the most resistant to sterilization?

bacteria
virus
fungi
prion
The smaller the agent, the more difficult it is to destroy it. Prions are quite resistant to proteases, heat, radiation, and formalin treatments. 134°C (274°F) for 18 minutes in a pressurized steam autoclave may not be enough to deactivate the agent of disease. Prions may last for decades in the environment.
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Answers by country

bacteria
virus
fungi
prion
answers
flagGermany
3%
12%
4%
80%
97
flagPakistan
12%
14%
7%
65%
123
flagRepublic of Ireland
6%
25%
2%
62%
43
flagItaly
17%
17%
4%
60%
23
flagGreece
11%
14%
14%
59%
27
flagPoland
9%
20%
10%
58%
19079
flagAustralia
6%
17%
12%
57%
63
flagthe Netherlands
14%
21%
10%
53%
28
flagMalaysia
14%
14%
19%
52%
42
flagUnknown
14%
20%
16%
50%
50
flagthe United Kingdom
14%
26%
11%
46%
302
flagthe United Arab Emirates
11%
26%
11%
46%
26
flagthe United States
12%
27%
13%
44%
885
flagSingapore
26%
26%
4%
43%
23
flagIndia
17%
27%
10%
42%
774
flagCanada
14%
31%
11%
42%
69
flagSouth Africa
19%
26%
3%
42%
26
flagGhana
17%
39%
0%
39%
28
flagthe Philippines
26%
22%
17%
32%
103
flagNigeria
6%
44%
13%
31%
45